Dorothy A Pierce

Title: Innovations by Dorothy A. Pierce

Introduction

Dorothy A. Pierce is a notable inventor based in Urbandale, IA (US). She has made significant contributions to the field of plant biotechnology, holding a total of 8 patents. Her work focuses on improving transformation techniques in maize and developing methods for producing transgenic plants.

Latest Patents

Among her latest patents, one includes methods for Agrobacterium-mediated transformation. This invention relates to enhancing the transformation frequency of maize embryos. The preferred method involves contacting immature maize embryos with Agrobacterium, co-cultivating them in a specific medium, and regenerating plants that express the desired gene. Another significant patent involves the use of green fluorescent protein (GFP) as a screenable marker for plant transformation. This method allows for the production of transgenic plants by introducing a vector carrying a gene encoding GFP into cells. The cells are then screened for the protein, and transformed cells are selected and regenerated. The invention also addresses the cellular toxicity of GFP by regulating its expression or directing it to non-toxic subcellular compartments.

Career Highlights

Dorothy A. Pierce has been associated with Pioneer Hi-Bred International, Inc., where she has contributed to advancements in agricultural biotechnology. Her innovative approaches have paved the way for more efficient plant transformation techniques.

Collaborations

She has collaborated with notable coworkers, including Zuo-Yu Zhao and Tishu Cai, to further enhance her research and development efforts in the field.

Conclusion

Dorothy A. Pierce's contributions to plant biotechnology through her patents and collaborations have significantly impacted the agricultural sector. Her innovative methods continue to influence the development of transgenic plants and improve agricultural practices.