Company Filing History:
Years Active: 1994
Title: The Innovative Contributions of Charles D. Puglia in Biochemical Assays
Introduction
Charles D. Puglia is a noted inventor based in Glenside, Pennsylvania, who has made significant strides in the field of biochemistry through his innovative work. His contributions primarily revolve around improving assay techniques that are crucial for understanding biochemical processes in living organisms.
Latest Patents
Puglia holds one patent for an invention titled "Inosinic Acid Dehydrogenase Assay." This patent outlines a cyto(histo)chemical technique aimed at determining the presence and concentration of inosine 5'-monophosphate dehydrogenase (IMPD) within various cell types classified from animal or human subjects. The technique involves preparing, fixing, and staining cell monolayers utilizing a specific procedural setup. Key components of this process include the enzyme substrate inosine 5'-monophosphate, nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS), and nitro blue tetrazolium (NBT). Together, these elements facilitate the creation of a granular, dark blue deposit of formazan in proportion to the IMPD activity present in the cells. The evaluation of these cells can be conducted visually or through advanced imaging systems, adhering to accepted biostatistical methods and standards.
Career Highlights
Charles D. Puglia’s professional journey is closely associated with the Medical College of Pennsylvania, where he has applied his expertise to advance the field of biochemical research. His innovative patent plays a critical role in understanding IMPD, which is vital for various physiological and pathological studies.
Collaborations
Throughout his career, Puglia has collaborated with prominent colleagues, including Olivera T. Markovic and Nenad S. Markovic. These collaborative efforts have contributed to furthering research in biochemistry and enhancing the impact of his patented techniques.
Conclusion
In conclusion, Charles D. Puglia's work and innovative patent in biochemical assays demonstrate his commitment to advancing scientific research. His contributions are invaluable to the understanding of enzymatic activities within cells, paving the way for further discoveries in the medical and biochemical fields.