Ce Ji

Title: Innovations in Genetic Research: The Contributions of Ce Ji

Introduction

Ce Ji is an innovative inventor based in Shanghai, China. He has made significant advancements in genetic research, focusing on the use of CRISPR/Cas9 technology to enhance our understanding of genetic functions and mutations. With a single patent to his name, Ji has already begun to make an impact in the field of molecular genetics.

Latest Patents

Ji's noteworthy patent is titled "Method of Constructing Zebrafish Mutants." This invention encompasses a novel technique for creating a zebrafish notch1a mutant utilizing the CRISPR/Cas9 method. The process begins with identifying a target site for knocking out notch1a. Ji details the use of specific primers, T7-notch1a-sfd and tracr rev, for PCR amplification with a pUC19-gRNA scaffold plasmid. After transcribing the PCR product in vitro and purifying it, the obtained gRNA is microinjected, alongside Cas9 mRNA, into a zebrafish embryo. This innovative method allows for the precise knockout of the notch1a gene, preserving the integrity of other genes and producing a stable notch1a mutant. Additionally, Ji's invention includes observations on the phenotypic attributes of the notch1a mutant, which are crucial for examining the Notch1a receptor's role within the Notch signaling pathway.

Career Highlights

Ce Ji's affiliation with Shanghai Ocean University is a significant aspect of his career. Here, he has been able to combine his passion for molecular biology with advanced research facilities. Through his role at the university, Ji has been able to further his studies and contribute to scientific advancements in genetic modification.

Collaborations

While working at Shanghai Ocean University, Ji collaborates with notable colleagues, including Qinghua Zhang. This partnership reflects a commitment to advancing genetic research and enhancing the understanding of complex biological systems.

Conclusion

Ce Ji exemplifies the spirit of innovation within the realm of genetic research. His pioneering patent method for constructing zebrafish mutants showcases the transformative potential of CRISPR/Cas9 technology in modern science. Through his work at Shanghai Ocean University and collaborative efforts with fellow researchers, Ji is contributing to a deeper understanding of genetic functions, underscoring the importance of innovation in shaping the future of biology.