Bert C Lampson

Title: Bert C. Lampson: Innovator in RNA-Linked DNA Synthesis

Introduction

Bert C. Lampson is a notable inventor based in Highland Park, NJ (US). He has made significant contributions to the field of molecular biology, particularly in the synthesis of branched RNA-linked multi-copy single-stranded DNA. His innovative work has implications for understanding genetic processes and developing new biotechnological applications.

Latest Patents

Lampson's patent focuses on the production of branched RNA-linked multi-copy single-stranded DNA using a unique molecule known as msDNA. This molecule consists of a branched RNA linked to single-stranded DNA via a 2', 5' phosphodiester bond. The patent describes a cell-free system that utilizes cells permeabilized with phenethyl alcohol to study the synthesis of msDNA in Myxococcus xanthus. The research demonstrated that permeabilized cells labeled with [.alpha.-.sup.32 P]dCTP in the presence of ddGTP, ddATP, or ddTTP produced a band that migrates at the same position as the full-sized msDNA in an acrylamide gel. However, treatment with ribonuclease A prior to gel electrophoresis resulted in multiple bands of different sizes, indicating the production of intermediates during labeling.

Career Highlights

Lampson has been associated with the University of Medicine and Dentistry of New Jersey, where he has conducted his research. His work has provided evidence for a model in which msDNA is synthesized by reverse transcriptase using a folded RNA precursor as both a primer and a template. Furthermore, he discovered a precise coupling mechanism between reverse transcriptase and ribonuclease H, which is crucial for the synthesis process.

Collaborations

Lampson has collaborated with notable researchers such as Masayori Inouye and Sumiko Inouye. Their joint efforts have contributed to advancing the understanding of RNA and DNA interactions, further enhancing the field of molecular biology.

Conclusion

Bert C. Lampson's innovative research in RNA-linked DNA synthesis has opened new avenues in molecular biology. His contributions are significant for both academic research and potential biotechnological applications.