Arlene A Wise

Title: Arlene A Wise: Innovator in Biosensor Technology

Introduction

Arlene A Wise is a notable inventor based in Philadelphia, PA (US). She has made significant contributions to the field of biosensor technology, particularly in the detection of phenols using engineered bacteria. With a total of 2 patents, her work has the potential to impact environmental monitoring and safety.

Latest Patents

Wise's latest patents focus on the development of biosensors that utilize engineered bacteria to detect phenols. The process involves placing a reporter gene under the control of an inducible promoter, which produces a signal when a cognate transcriptional activator senses the inducing chemical. The creation of bacterial biosensors has been limited by the restricted knowledge of the genetic systems of bacteria that catabolize xenobiotics. By employing mutagenic PCR to modify the chemical specificity of the Pseudomonas species CF600 DmpR protein, Wise has demonstrated the potential for engineering novel biosensors for phenol detection. The DmpR protein, a well-characterized transcriptional activator of the P. CF600's dmp operon, mediates growth on simple phenols. The transcription from the promoter Po, which heads the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of DmpR, Wise has created a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols. The assays and sensor domain mutations that modify the chemical specificity of DmpR are thoroughly described in her patents.

Career Highlights

Throughout her career, Arlene A Wise has worked with prestigious institutions, including the University of California and Los Alamos National Security, LLC. Her innovative research has positioned her as a leader in the field of biosensor technology.

Collaborations

Wise has collaborated with notable colleagues, including Cheryl R Kuske and Thomas C Terwilliger, contributing to the advancement of her research and the development of new technologies.

Conclusion

Arlene A Wise's contributions to biosensor technology and her innovative approach to detecting phenols using engineered bacteria highlight her as a significant figure in the field. Her work not only advances scientific knowledge but also has practical applications in environmental monitoring.