Andrew M Tometsko

Title: Innovations of Andrew M Tometsko

Introduction

Andrew M Tometsko is an accomplished inventor based in Rochester, NY. He has made significant contributions to the field of genetic toxicology and cleaning solutions, holding two patents that showcase his innovative spirit and dedication to improving health and safety.

Latest Patents

One of his latest patents is a process for analyzing clastogenic agents. Genotoxic chemicals pose a widespread health hazard to the human population. Advances in genetic toxicology testing have enabled the assessment of potential mutagens, carcinogens, teratogens, and clastogens in the environment. The mouse micronucleus assay serves as an excellent test for genetic damage to cells. When chromosome breaks occur in the blood stem cell population, the damaged chromosome piece remains as a micronucleus in the normally DNA deficient red blood cells. However, existing manual micronucleus assays are costly, time-consuming, and labor-intensive. This invention discloses a method for assaying changes in micronucleated cells using high-speed flow cytometry. In this procedure, cells are streamed at high speed through a laser beam, allowing for the collection of fluorescence emission and light scatter properties of each cell. The invention details the procedures for dosing mice, obtaining blood samples, fixing and staining cells, configuring the flow cytometer for MN analysis, and modes of data acquisition and analysis. Internal and external quality controls are also described to optimize flow cytometer conditions and evaluate data fidelity in real time. This process allows for the analysis of 1,000,000 total cells for each sample in minutes, significantly improving statistics and assay sensitivity.

His second patent involves a liquid cleaner containing inactivated protease for protein-soiled contact lenses. This innovative solution conveniently cleans contact lenses by using an aqueous solution that contains an inactivated sulfhydryl protease and a mild thio reducing agent. The protease is allosterically inactivated through a reaction with sodium tetrathionate.

Career Highlights

Throughout his career, Andrew has worked with various companies, including Litron Laboratories Ltd. His work has had a lasting impact on the fields of genetic toxicology and cleaning solutions.

Collaborations

Andrew has collaborated with notable individuals such as Subir Chanda and Thomas M Riedhammer, contributing to the advancement of his innovative projects.

Conclusion

Andrew M Tometsko's contributions to innovation in genetic toxicology and cleaning