The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 12, 2018
Filed:
Jun. 29, 2012
Minna Nyström, Helsingin yliopisto, FI;
Minttu Kansikas, Helsingin yliopisto, FI;
Päivi Peltomäki, Helsingin yliopisto, FI;
Minna Nyström, Helsingin yliopisto, FI;
Minttu Kansikas, Helsingin yliopisto, FI;
Päivi Peltomäki, Helsingin yliopisto, FI;
HELSINGIN YLIOPISTO, Helsingin Yliopisto, FI;
Abstract
This invention relates to a quantitative method for determining whether a human subject has an impaired DNA mismatch repair function; providing a diagnostic sample taken from said human and producing a nuclear extract from said sample; providing MMR proficient and MMR deficient nuclear extracts as positive and negative controls, respectively; combining each nuclear extract with at least one mismatch bearing substrate DNA molecule; performing a mismatch repair assay; and determining whether said sample nuclear extract is capable of repairing said substrate DNA molecule; wherein said sample comprises normal, non-malignant constitutive cells, such as fibroblasts. The invention further relates to a kit providing necessary reagents for use in said method.