The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 01, 2018
Filed:
Jun. 30, 2011
Jian LI, Guangdong, CN;
Shiping Chen, Guangdong, CN;
Xiandong Zhang, Guangdong, CN;
Ying Liu, Guangdong, CN;
Caifen Zhang, Guangdong, CN;
Tao Liu, Guangdong, CN;
Meiru Zhao, Guangdong, CN;
Jian Li, Guangdong, CN;
Shiping Chen, Guangdong, CN;
Xiandong Zhang, Guangdong, CN;
Ying Liu, Guangdong, CN;
Caifen Zhang, Guangdong, CN;
Tao Liu, Guangdong, CN;
Meiru Zhao, Guangdong, CN;
BGI Genomics Co., Ltd., Shenzhen, CN;
Abstract
The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer while making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.