The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 20, 2018

Filed:

Apr. 27, 2010
Applicants:

Seung Goo Lee, Daejeon, KR;

Jae Seok Ha, Daejeon, KR;

Jae Jun Song, Daejeon, KR;

Inventors:

Seung Goo Lee, Daejeon, KR;

Jae Seok Ha, Daejeon, KR;

Jae Jun Song, Daejeon, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/542 (2006.01); B82Y 15/00 (2011.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/542 (2013.01); B82Y 15/00 (2013.01); G01N 33/582 (2013.01); G01N 33/587 (2013.01); G01N 33/588 (2013.01); G01N 2500/00 (2013.01);
Abstract

The present application relates to a method for detecting ligand using a biosensor applied the FRET (fluorescence resonance energy transfer) phenomenon. More particularly, the method may be used for simply detecting a ligand in a sample by measuring the FRET of a biosensor under the conditions in which a specific critical temperature is maintained. The method may use a phenomenon in which a ligand-binding protein in a biosensor shows reversible unfolding at a temperature higher than the specific critical temperature and the level of the unfolding changes depending on the concentration of a ligand. The method can be widely applied to a variety of kinds of FRET biosensors using the ligand-binding protein.


Find Patent Forward Citations

Loading…