The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 13, 2018
Filed:
Mar. 28, 2016
Hai Xing Chen, Toronto, CA;
Hai Xing Chen, Toronto, CA;
ACGT Corporation, Toronto, CA;
Abstract
A method of enhancing signal in detection of nucleic acid sequences utilizes dual-functional capture particles for capturing a target sequence and for binding with enhancement probes coupled to a reporter. The method includes hybridizing a target sequence with a capture sequence on dual-functional capture particles; hybridizing a pair of enhancement probes to the bounded target sequence through a linker to form a staggered chain of enhancement probes; triggering a chemical reaction between a chemical binding group on the dual-functional capture particles and a first binding moiety on the enhancement probes to bind the enhancement probes covalently to dual-functional capture particles; binding a reporter to the bounded enhancement probes through another chemical reaction between a second binding moiety on the enhancement probes and a binding functional group on the reporter; and detecting the signal from a signal generating molecule on bounded reporter, thereby amplifying signal through enhancement probes coupled to reporters.