The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 2018
Filed:
Feb. 12, 2014
The Regents of the University of Colorado, Denver, CO (US);
The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US);
Rafael Piestun, Boulder, CO (US);
Sri Rama Prasanna Pavani, Pasadena, CA (US);
Michael A. Thompson, Orange, CA (US);
Julie S. Biteen, Menlo Park, CA (US);
William E. Moerner, Los Altos, CA (US);
THE REGENTS OF THE UNIVERSITY OF COLORADO, Denver, CO (US);
Abstract
Embodiments of the present invention can resolve molecules beyond the optical diffraction limit in three dimensions. A double-helix point spread function can be used to in conjunction with a microscope to provide dual-lobed images of a molecule. Based on the rotation of the dual-lobed image, the axial position of the molecule can be estimated or determined. In some embodiments, the angular rotation of the dual-lobed imaged can be determined using a centroid fit calculation or by finding the midpoints of the centers of the two lobes. Regardless of the technique, the correspondence between the rotation and axial position can be utilized. A double-helix point spread function can also be used to determine the lateral positions of molecules and hence their three-dimensional location.