The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 02, 2018

Filed:

May. 27, 2014
Applicant:

Carl Zeiss Microscopy Gmbh, Jena, DE;

Inventors:

Jorg Engel, Weida, DE;

Thomas Kalkbrenner, Jena, DE;

Wolfgang Bathe, Jena, DE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
H04N 7/18 (2006.01); G02B 21/36 (2006.01); G02B 21/16 (2006.01); G02B 27/58 (2006.01); G01N 21/64 (2006.01); G02B 21/26 (2006.01); G06T 7/77 (2017.01); G06T 7/536 (2017.01);
U.S. Cl.
CPC ...
G02B 21/367 (2013.01); G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G01N 21/6486 (2013.01); G02B 21/16 (2013.01); G02B 21/26 (2013.01); G02B 27/58 (2013.01); G06T 7/536 (2017.01); G06T 7/77 (2017.01); G06T 2200/04 (2013.01); G06T 2207/10024 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/10072 (2013.01);
Abstract

The invention relates to a method for high-resolution luminescence microscopy of a specimen marked with marker molecules, and to a luminescence microscope for performing the method, wherein the marker molecules can be excited to emit luminescence radiation. The method for luminescence microscopy comprises the excitation and imaging of marker molecules and the transmission of a trigger time and a position of the specimen. An optical recording device images the marker molecules in a capture area and transmits data from the imaging to an image capture circuit. The recording device transmits a time for the imaging to a signal former as a trigger time; the trigger time is then transmitted to a data recorder. The data recorder generates a position of the specimen at the trigger time and transmits said position to the image capture circuit, which links the position of the specimen in the depth direction to the data of the imaging of a frame such that a three-dimensional tomographic image of the specimen can be created.


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