The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 05, 2017

Filed:

Mar. 30, 2015
Applicant:

Board of Regents, the University of Texas System, Austin, TX (US);

Inventors:

Steven G. Widen, Galveston, TX (US);

Thomas G. Wood, Houston, TX (US);

Allan R. Brasier, Galveston, TX (US);

Yingxin Zhao, Houston, TX (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/00 (2006.01); A61K 49/00 (2006.01); A61K 47/48 (2006.01); A61K 31/7088 (2006.01); C12N 15/115 (2010.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C12N 15/115 (2013.01); A61K 31/7088 (2013.01); A61K 47/48092 (2013.01); A61K 49/0002 (2013.01); G01N 33/5308 (2013.01); C12N 2310/16 (2013.01); C12N 2310/3517 (2013.01); G01N 2333/47 (2013.01);
Abstract

DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.


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