The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2017
Filed:
Jun. 13, 2011
Masaaki Sawa, Ibaraki, JP;
Chi-huey Wong, Taipei, TW;
Tsui-ling Hsu, Taipei, TW;
Sarah Hanson, San Marcos, CA (US);
Masaaki Sawa, Ibaraki, JP;
Chi-Huey Wong, Taipei, TW;
Tsui-Ling Hsu, Taipei, TW;
Sarah Hanson, San Marcos, CA (US);
ACADEMIA SINICA, Taipei, TW;
Abstract
Methods for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as 'tags' into cellular glycoconjugates are disclosed. Alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars are incorporated into cellular glycoconjugates. Chemical probes comprising an azide group and a visual or fluorogenic probe and used to label alkyne-derivatized sugar-tagged glycoconjugates are disclosed. Chemical probes bind covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and are visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA, confocal microscopy, and mass spectrometry.