The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2017
Filed:
Oct. 10, 2008
Sergei Saveliev, Madison, WI (US);
Daniel Simpson, Middleton, WI (US);
Keith V. Wood, Mt. Horeb, WI (US);
Harry Tetsuo Uyeda, Los Osos, CA (US);
Carolyn Woodroofe Hitko, Grover Beach, CA (US);
Dieter Klaubert, Arroyo Grande, CA (US);
Sergei Saveliev, Madison, WI (US);
Daniel Simpson, Middleton, WI (US);
Keith V. Wood, Mt. Horeb, WI (US);
Harry Tetsuo Uyeda, Los Osos, CA (US);
Carolyn Woodroofe Hitko, Grover Beach, CA (US);
Dieter Klaubert, Arroyo Grande, CA (US);
PROMEGA CORPORATION, Madison, WI (US);
Abstract
The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.