The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2017
Filed:
Sep. 08, 2016
The United States of America, As Represented BY the Secretary of Agriculture, Washington, DC (US);
Manuel V. Borca, Westbrook, CT (US);
Douglas P. Gladue, Guilford, CT (US);
Lauren G. Holinka-Patterson, Deep River, CT (US);
Vivian O'Donnell, Old Saybrook, CT (US);
Jose Nieva, Bilboa, ES;
The United States of America, as represented by The Secretary of Agriculture, Washington, DC (US);
The University of Connecticut, Farmington, CT (US);
Universidad del Pais Vasco/Euskal Herriko Univertsitatea (UPV-EHU), , ES;
Abstract
The role of a specific E2 region containing a putative fusion peptide (FP) sequence was evaluated. FPs critically contribute to the interaction between proteins and the membrane system of the host cell. Reverse genetics utilizing a full-length infectious clone of the highly virulent CSFV strain Brescia (BICv) was used to evaluate how amino acid substitutions within this region of E2 may affect replication of BICv in cell cultures and affect virus virulence in swine. Interestingly, mutated virus FPi.c was completely attenuated when inoculated intranasally at a dose of 10TCID50 in swine. Importantly, animals infected with FPi.c virus were protected against the virulent challenge with Brescia virus at 3 and 28 days after vaccination. Protection was evidenced by absence of clinical signs related with CSF as well as the absence of viremia produced by the challenge virulent virus.