The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2017

Filed:

Aug. 25, 2014
Applicant:

454 Life Sciences Corporation, Branford, CT (US);

Inventors:

Hideji Tajima, Matsudo, JP;

Tetsuya Ueda, Matsudo, JP;

Shuichi Kobayashi, Matsudo, JP;

Nathan Robert Kane, Guilford, CT (US);

Assignee:

454 Life Sciences Corporation, Branford, CT (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 7/00 (2006.01); B01F 7/00 (2006.01); B01F 11/00 (2006.01); B01L 3/00 (2006.01); B01F 3/08 (2006.01); C12Q 1/68 (2006.01); B01L 3/02 (2006.01); G01N 35/00 (2006.01);
U.S. Cl.
CPC ...
B01L 7/52 (2013.01); B01F 3/0807 (2013.01); B01F 7/00116 (2013.01); B01F 7/00141 (2013.01); B01F 7/00725 (2013.01); B01F 11/0088 (2013.01); B01L 3/508 (2013.01); B01L 3/0293 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/1822 (2013.01); C12Q 1/686 (2013.01); G01N 2035/00534 (2013.01); G01N 2035/00891 (2013.01);
Abstract

An embodiment of a device for automatically executing a process of generating an emulsion containing nucleic acids, amplifying the nucleic acids in the emulsion, breaking the emulsion, and separating and purifying said amplified nucleic acids, is described that comprises an emulsion generation unit for sealing beads to which nucleic acids are bound in a water-in-oil type emulsion; a nucleic acid amplification unit provided with a reaction vessel for amplifying said nucleic acids and a heating and cooling part for heating and cooling the reaction vessel; an emulsion breaking unit for breaking the emulsion after nucleic acid amplification; and a nucleic acid purification unit for recovering said amplified nucleic acids from said emulsion breaking unit.


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