The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2017
Filed:
Jan. 08, 2016
Life Technologies Corporation, Carlsbad, CA (US);
Peter Vander Horn, Encinitas, CA (US);
Cheng-Yao Chen, Carlsbad, CA (US);
Guobin Luo, Oceanside, CA (US);
Michael Previte, Carlsbad, CA (US);
Jamshid Temirov, Germantown, TN (US);
Theo Nikiforov, Carlsbad, CA (US);
Zhaohui Zhou, San Ramon, CA (US);
Hongye Sun, Belmont, CA (US);
Yufang Wang, San Carlos, CA (US);
Stefanie Yukiko Nishimura, Mountain View, CA (US);
Hongyi Wang, Pearland, TX (US);
Marian Peris, Belmont, CA (US);
Barnett Rosenblum, San Jose, CA (US);
Michael Phelan, Hayward, CA (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.