The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 12, 2017
Filed:
Aug. 28, 2009
Thomas Jostock, Neuenburg am Rhein, DE;
Hans-peter Knopf, Schallstadt, DE;
Burkhard Wilms, Weil am Rhein, DE;
Audrey Nommay, Blotzheim, FR;
Thomas Jostock, Neuenburg am Rhein, DE;
Hans-Peter Knopf, Schallstadt, DE;
Burkhard Wilms, Weil am Rhein, DE;
Audrey Nommay, Blotzheim, FR;
Novartis AG, Basel, CH;
Abstract
The application describes a method of selecting mammalian host cells that express a polypeptide of interest with high yield. The host cells contain an expression cassette with a first polynucleotide encoding a polypeptide of interest, at least one leaky stop codon located downstream of the first polynucleotide and a second polynucleotide located downstream of the leaky codon encoding an immunoglobulin transmembrane anchor comprising a cytoplasmic domain. The host cells are cultivated to allow expression of the polypeptide of interest such that some of the polypeptides of interest are expressed as fusion proteins displayed on the cell surface. High producing cells are then selected based on the presence or amount of the displayed fusion polypeptides.