The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 22, 2017

Filed:

Aug. 16, 2013
Applicants:

The Scripps Research Institute, La Jolla, CA (US);

Hongkai Zhang, San Diego, CA (US);

Ian A. Wilson, La Jolla, CA (US);

Richard A. Lerner, La Jolla, CA (US);

Inventors:

Hongkai Zhang, San Diego, CA (US);

Ian A. Wilson, La Jolla, CA (US);

Richard A. Lerner, La Jolla, CA (US);

Assignee:

The Scripps Research Institute, La Jolla, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/06 (2006.01); C07K 16/28 (2006.01); G01N 33/68 (2006.01); G01N 33/74 (2006.01); C07K 14/71 (2006.01); C07K 14/715 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2863 (2013.01); C07K 14/71 (2013.01); C07K 14/7153 (2013.01); C07K 16/2839 (2013.01); C07K 16/2866 (2013.01); G01N 33/6845 (2013.01); G01N 33/6854 (2013.01); G01N 33/746 (2013.01); C07K 2317/21 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/622 (2013.01); C07K 2317/74 (2013.01); C07K 2317/75 (2013.01); C07K 2317/92 (2013.01); C07K 2319/30 (2013.01); C40B 30/06 (2013.01);
Abstract

Methods for Identifying protein modulators of eukaryotic cells by expressing a combinatorial library of potential agonists inside a eukaryotic cell and then directly selecting for an agonist of a target molecule. Some methods involve co-culturing a cell expressing a combinatorial library of potential agonists and a second cell, and then selecting agents that modulate a phenotype of or a desired cellular response in the second cell. Preferably, the agonists are antibodies introduced into and expressed in the starting cells, such as agonist anti-EpoR, anti-TpoR, or G-CSFR antibodies. Also disclosed are methods for selecting from combinatorial antibody libraries bispecific antibodies that can regulate cell phenotypes.


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