The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2017
Filed:
Feb. 03, 2015
Applicant:
Nature Technology Corporation, Lincoln, NE (US);
Inventors:
James A. Williams, Lincoln, NE (US);
Clague P. Hodgson, Omaha, NE (US);
Aaron E. Carnes, Lincoln, NE (US);
Assignee:
Nature Technology Corporation, Lincoln, NE (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/64 (2006.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01); C12N 15/73 (2006.01); C12P 19/34 (2006.01); C12N 1/21 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12N 15/64 (2013.01); C12N 1/06 (2013.01); C12N 15/1003 (2013.01); C12N 15/1017 (2013.01); C12N 15/73 (2013.01);
Abstract
General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.