The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 25, 2017
Filed:
Nov. 22, 2012
Hitachi High-technologies Corporation, Tokyo, JP;
Hitachi High-Technologies Corporation, Tokyo, JP;
Abstract
A nucleic acid amplification method includes ligating a double-stranded adapter () containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA () containing a target DNA sequence () to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (). A 3'-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick () as an origin, thereby producing a concatemer () in which a plurality of the target DNA sequences () and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer () contains a plurality of the target DNA sequences () suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.