The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 13, 2017
Filed:
Mar. 13, 2013
Omeros Corporation, Seattle, WA (US);
University of Washington Through Its Center for Commercialization, Seattle, WA (US);
Daniel S. Allison, Lake Forest Park, WA (US);
W. Jason Cummings, Bellevue, WA (US);
John B. Leppard, Mukilteo, WA (US);
Nancy Maizels, Seattle, WA (US);
Larry W. Tjoelker, Kirkland, WA (US);
Christi L. Wood, Snohomish, WA (US);
Munehisa Yabuki, Seattle, WA (US);
Omeros Corporation, Seattle, WA (US);
University of Washington Through Its Center for Commercialization, Seattle, WA (US);
Abstract
The disclosure relates generally to the targeting of genes to, and their integration into, an immunoglobulin (antibody) heavy chain locus. In particular, the methods described herein contemplate replacing the single rearranged heavy chain V, D, and J genes of a B cell lymphoma such as DT40 with independently rearranged V-D-Jgenes of chicken, in a system for generating immunoglobulin diversity. Also contemplated is replacement of the chicken V-D-Jwith rearranged V-D-Jgenes of other vertebrates including human in a system for generating immunoglobulin diversity, with the exception of any substitution disclosed and claimed in PCT application WO 2009/029315 A2. Also described is construction of a diverse chicken immunoglobulin heavy chain VDJ library in DT40 by homologous gene replacement of the single endogenous rearranged VDJ gene with a chicken VDJ repertoire using the described targeting vectors.