The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9676817 B1

PDF
Full Text
Expired
Date of Patent:
Jun. 13, 2017

Filed:

Dec. 19, 2012
Applicant:

Novozymes A/s, Bagsvaerd, DK;

Inventors:

Poul Johansen, Virum, DK;

Cecilia Jansson Kepka, Bunkeflostrand, SE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/36 (2006.01); C07K 1/30 (2006.01); A61L 2/00 (2006.01); C07K 1/00 (2006.01);
U.S. Cl.
CPC ...
C07K 1/30 (2013.01); A61L 2/0011 (2013.01);
Abstract

The author attempted and succeeded in separating and purifying the cysteine protease inhibitors from new gingers. The root and stem of new gingers were extracted with neutral buffer which subsequently underwent heat treatment at 80° C. for 10 minutes. After centrifugal separation, the supernatant was separated with acetone. The fractions that precipitated at an acetone concentration of 55-77% were injected into a chromatographic column of DEAE-cellulose and Sephadex G-75 gel for purification. The results demonstrated that there were two cysteine protease inhibitors with a molecular weight of 11,000-11,800 and 15,500-16,000, respectively, which strongly inhibit papain. The two cysteine protease inhibitors do not have high heat stability.


Find Patent Forward Citations

Johansen, Poul. (2017). Method for the removal of virus from a protein solution (U.S. Patent No. 9676817). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9676817

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