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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9657301 B1

PDFFull Text
Date of Patent:
May. 23, 2017

Filed:

Dec. 28, 2015

Filamentous fungal mutants with improved homologous recombination efficiency

Applicant:

Dsm Ip Assets B.v., Heerlen, NL;

Inventors:

Petrus Jacobus Theodorus Dekker, Echt, NL;

Marco Alexander van den Berg, Echt, NL;

Assignee:

DSM IP ASSETS B.V., Heerlen, NL;

Attorney:

McBee Moore Woodward & Vanik IP, LLC

Primary Examiner:

Yong Pak

Int. Cl.
CPC ...
C12N 1/14 (2006.01); C12N 15/00 (2006.01); C12Q 1/68 (2006.01); C12N 9/00 (2006.01); C12P 21/04 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01); C12N 15/80 (2006.01); C07K 14/37 (2006.01); C12N 15/90 (2006.01); C12P 21/02 (2006.01); C12P 23/00 (2006.01); C12P 37/00 (2006.01); C12P 17/10 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/80 (2013.01); C07K 14/37 (2013.01); C12N 15/902 (2013.01); C12P 17/10 (2013.01); C12P 21/00 (2013.01); C12P 21/02 (2013.01); C12P 23/00 (2013.01); C12P 37/00 (2013.01);
Abstract

The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.

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