The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 09, 2017
Filed:
Sep. 24, 2009
Ezra Abrams, Newton, MA (US);
Sadanand Gite, Arlington, MA (US);
Lisa Shinefeld, Lexington, MA (US);
Don Straus, Cambridge, MA (US);
Gordon Siek, Somerville, MA (US);
Greg Yantz, Somerville, MA (US);
Ezra Abrams, Newton, MA (US);
Sadanand Gite, Arlington, MA (US);
Lisa Shinefeld, Lexington, MA (US);
Don Straus, Cambridge, MA (US);
Gordon Siek, Somerville, MA (US);
Greg Yantz, Somerville, MA (US);
First Light Biosciences, Inc., Bedford, MA (US);
Abstract
The invention provides an improved method for sensitive and specific detection of target molecules, cells, or viruses. The inventive method uses large area imaging to detect individual labeled targets complexed with a target-specific selection moiety. The invention eliminates wash steps through the use of target-specific selection through one or more liquid layers that can contain optical dye and density agents. By eliminating washes the invention simplifies instrumentation engineering and minimizes user steps and costs. The invention uses sensitive image analysis to enumerate individual targets in a large area, is scalable, and can be deployed in systems ranging in complexity from manual to highly automated.