The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2017

Filed:

Aug. 22, 2014
Applicant:

454 Life Sciences Corporation, Branford, CT (US);

Inventors:

Brian Christopher Godwin, North Haven, CT (US);

Priya Shanbhag, Rocky Hill, CT (US);

Craig Elder Mealmaker, Jersey City, NJ (US);

Gianni Calogero Ferreri, Northford, CT (US);

Melinda Palmer, Hamden, CT (US);

Shally Hsueh-Wen Wang, Guilford, CT (US);

Assignee:

454 Life Sciences Corporation, Branford, CT (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6869 (2013.01); C12Q 2525/191 (2013.01); C12Q 2565/518 (2013.01);
Abstract

An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule.


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