The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 18, 2017
Filed:
May. 18, 2012
Takashi Nonaka, Tokyo, JP;
Masami Masuda, Tokyo, JP;
Makiko Yamashita, Tokyo, JP;
Haruhiko Akiyama, Tokyo, JP;
Masato Hasegawa, Tokyo, JP;
Takashi Nonaka, Tokyo, JP;
Masami Masuda, Tokyo, JP;
Makiko Yamashita, Tokyo, JP;
Haruhiko Akiyama, Tokyo, JP;
Masato Hasegawa, Tokyo, JP;
TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, Tokyo, JP;
Abstract
The purpose of the present invention is to develop a method for amplifying in vitro to a large amount of a homogenous insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient. A method of producing an insoluble aggregate including TDP-43 protein and fragments thereof according to the present invention includes the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cell culture in which the intact TDP-43 protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) separating an insoluble fraction from the cultured cell. Optionally, the method may additionally include a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell.