The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 14, 2017
Filed:
May. 21, 2014
Src, Inc., North Syracuse, NY (US);
Jeffrey H. Mills, Liverpool, NY (US);
Huda S. Suliman, North Syracuse, NY (US);
Stacey A. Massulik, Syracuse, NY (US);
Frances L. Stites, Ashburn, VA (US);
Timothy F. Moshier, Fulton, NY (US);
Kenton A. Doctor, East Syracuse, NY (US);
Lisa H. Chamberlin, East Syracuse, NY (US);
Justin P. Andrews, Camillus, NY (US);
Stephen R. Houghton, Tully, NY (US);
SRC, INC., North Syracuse, NY (US);
Abstract
A kit and method for detection of a target in a sample. An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a second epitope of the target and conjugated to a DNA oligonucleotide; a reverse primer with a first region complimentary to the RNA oligonucleotide and a second region complimentary to the DNA oligonucleotide; and a reverse transcriptase that creates a DNA transcription product from the RNA oligonucleotide using the reverse primer only if the RNA oligonucleotide and the DNA oligonucleotide are in close proximity. If the target is present in the sample, the reverse primer binds the RNA oligonucleotide and the DNA oligonucleotide to bring the RNA oligonucleotide and the DNA oligonucleotide in close proximity.