The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 14, 2017
Filed:
Feb. 26, 2015
Applicants:
Yi Yang, Belmont, MA (US);
Marcello Maresca, Cambridge, MA (US);
Inventors:
Yi Yang, Belmont, MA (US);
Marcello Maresca, Cambridge, MA (US);
Assignee:
Novartis AG, Basel, CH;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 15/10 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12N 15/90 (2013.01); C12N 15/102 (2013.01); C12N 15/85 (2013.01); A01K 2217/00 (2013.01); C12N 2800/80 (2013.01);
Abstract
The present invention relates to genetic techniques employing the direct ligatation of an external DNA fragment generated in situ by the same ZFNs that target the genome. ObLiGaRe, i.e., the obligated ligation-gated recombination, is a new method for genetic engineering using custom designed nucleases, and a strategy of site-specific gene insertion utilizing the NHEJ pathway. It applies a similar logic to the one used in unidirectional loxP sites (Oberdoerffer et al., 2003) but maintains all the advantages and flexibility of CDNs.