The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 31, 2017

Filed:

Sep. 05, 2013
Applicant:

University of Rochester, Rochester, NY (US);

Inventors:

Rudi Fasan, Rochester, NY (US);

John R. Frost, Niagara Falls, NY (US);

Assignee:

University of Rochester, Rochester, NY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C40B 40/10 (2006.01); C12P 21/04 (2006.01); A61K 47/48 (2006.01); C08G 63/48 (2006.01); C08G 63/91 (2006.01); C08L 89/00 (2006.01); A61K 38/00 (2006.01); C07K 5/00 (2006.01); C07K 7/00 (2006.01); C07K 16/00 (2006.01); C07K 17/00 (2006.01); C07D 235/02 (2006.01); C07D 311/02 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01); C07D 495/04 (2006.01); C09B 57/02 (2006.01); C12N 9/42 (2006.01); A61K 49/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/582 (2013.01); A61K 47/48338 (2013.01); A61K 49/0039 (2013.01); A61K 49/0054 (2013.01); C07D 495/04 (2013.01); C09B 57/02 (2013.01); C12N 9/2442 (2013.01); G01N 33/6803 (2013.01); C07K 2319/20 (2013.01); C07K 2319/21 (2013.01); C07K 2319/50 (2013.01); C07K 2319/60 (2013.01); C07K 2319/92 (2013.01);
Abstract

Methods and compositions are provided for covalently linking a chemical species to a recombinant or synthetic polypeptide. The methods involve the reaction of a thioester-comprising polypeptide with a reagent comprising a reactive amino-thiol group connected to the chemical species which is to be covalently linked to the polypeptide, via a linker. Such chemical species can be a functional group, a label or tag molecule, a biological molecule, a ligand, or a solid support. Efficient and catalyst-free methods for C-terminal protein labeling are also provided. The methods expand current capabilities in the area of protein functionalization, providing useful and complementary tools for the isolation, detection, characterization, and analysis of proteins in a variety of in vitro and in vivo applications.


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