The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 22, 2016
Filed:
Aug. 10, 2012
Applicants:
Shinya Yamanaka, Kyoto, JP;
Keisuke Okita, Kyoto, JP;
Inventors:
Shinya Yamanaka, Kyoto, JP;
Keisuke Okita, Kyoto, JP;
Assignee:
KYOTO UNIVERSITY, Kyoto, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 5/074 (2010.01); C12N 15/79 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 15/79 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2506/02 (2013.01); C12N 2506/13 (2013.01); C12N 2506/1307 (2013.01); C12N 2506/1361 (2013.01); C12N 2510/00 (2013.01);
Abstract
A method of producing an induced pluripotent stem cell includes introducing into a somatic cell one or more non-viral expression vectors. The vectors include one or more of an Oct family gene, a Klf family gene, a Sox family gene, a Myc family gene, a Lin family gene, and Nanog gene. The somatic cell is then cultured in a medium that supports pluripotent stem cells. At least a portion of the one or more introduced non-viral expression vectors is not substantially integrated in the chromosome.