The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2016

Filed:

Mar. 31, 2006
Applicants:

Tomoyuki Igawa, Shizuoka, JP;

Hiroyuki Tsunoda, Shizuoka, JP;

Akiko Koga, Tokyo, JP;

Yasufumi Kikuchi, Shizuoka, JP;

Inventors:

Tomoyuki Igawa, Shizuoka, JP;

Hiroyuki Tsunoda, Shizuoka, JP;

Akiko Koga, Tokyo, JP;

Yasufumi Kikuchi, Shizuoka, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/00 (2006.01); C12P 21/08 (2006.01); A61K 39/00 (2006.01); A61K 51/10 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2866 (2013.01); C07K 2317/622 (2013.01); C07K 2317/626 (2013.01); C07K 2317/75 (2013.01);
Abstract

Structural isomers in sc(Fv)2 compositions of anti-human Mpl antibody and humanized anti-human Mpl antibody were separated, and the obtained structural isomers were cleaved at their linkers to confirm that the structural isomers are of single chain diabody type and bivalent scFv type. In addition, the agonistic activities of these structural isomers were revealed to be significantly different. Furthermore, the present inventors discovered that the content ratio of the structural isomers in sc(Fv)2 compositions could be regulated by altering temperature, modifying lengths of the linkers of sc(Fv)2, or amino acids in their variable regions.


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