The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2016
Filed:
Apr. 14, 2012
Scott C. Blanchard, New York, NY (US);
Michael Brian Feldman, Setauket, NY (US);
Leyi Wang, New York, NY (US);
James H. Doudna Cate, Berkeley, CA (US);
Arto Pulk, Berkeley, CA (US);
Roger B. Altman, New York, NY (US);
Michael R. Wasserman, New York, NY (US);
Scott C. Blanchard, New York, NY (US);
Michael Brian Feldman, Setauket, NY (US);
Leyi Wang, New York, NY (US);
James H. Doudna Cate, Berkeley, CA (US);
Arto Pulk, Berkeley, CA (US);
Roger B. Altman, New York, NY (US);
Michael R. Wasserman, New York, NY (US);
Cornell University, Ithaca, NY (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
The present invention relates to methods to identify molecules that binds in the neomycin binding pocket of a bacterial ribosome using structures of an intact bacterial ribosome that reveal how the ribosome binds tRNA in two functionally distinct states, determined by x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor (RRF) and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit (P/E) site. Additionally, the invention relates to various assays, including single-molecule assay for ribosome recycling, and methods to identify compounds that interfere with ribosomal function by detecting newly identified intermediate FRET states using known and novel FRET pairs on the ribosome. The invention also provides vectors and compositions with an N-terminally tagged S13 protein.