The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 16, 2016
Filed:
Nov. 28, 2014
Research & Business Foundation Sungkyunkwan University, Suwon-si, KR;
Suk Tae Kwon, Suwon-si, KR;
Sung Suk Cho, Suwon-si, KR;
Mi Yu, Suwon-si, KR;
Kyung Min Kwon, Suwon-si, KR;
Seung Hyun Kim, Suwon-si, KR;
Research & Business Foundation SUNGKYUNKWAN UNIVERSITY, Suwon-si, KR;
Abstract
A DNA polymerase (Neq DNA polymerase) derived fromis split into Neq L and Neq S fragments, each of which contains inteins. A Neq hot-start (HS) DNA polymerase in which the inteins of the Neq L and Neq S fragments are linked with each other is provided in the form of a precursor of Neq DNA polymerase. A purification method can be significantly improved by inserting a His-tag sequence composed of six histidine residues between the inteins of the Neq L and Neq S fragments at a gene level. As a result of effort to enhance PCR efficiency of the Neq HS DNA polymerase, a gene coding for the Neq HS DNA polymerase is mutated at specific positions to screen mutant Neq HS polymerases (M1, M2, and M3) having a highly improved PCR amplification rate and amplification level.