The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 09, 2016
Filed:
Sep. 25, 2014
Research and Diagnostic Systems, Inc., Minneapolis, MN (US);
Zhengliang L. Wu, Edina, MN (US);
Bio-Techne Corporation, Minneapolis, MN (US);
Abstract
Methods and kits for detecting the presence of GlcNAc modification of a peptide or protein. The steps of detection may include combining the peptide or protein with PAPS and a sulfotransferase to produce a reaction product includingS labeled GlcNAc modified protein or peptide, removing background labeling with a glycosidase, separating theS labeled GlcNAc modified protein or peptide from PAPS, free S-35 sulfate and labeled oligosaccharides if present, and detecting isotopic emissions from theS labeled GlcNAc modified peptide or protein. The detection may further include, prior to detection, the step of combining the peptide or protein with a GlcNAc transferase and UDP-GlcNAc under appropriate conditions for the GlcNAc transferase to transfer a GlcNAc to the peptide or protein.