The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 02, 2016

Filed:

Oct. 12, 2012
Applicant:

ES Cell International Pte Ltd, Alameda, CA (US);

Inventors:

Bruce Paul Davidson, Singapore, SG;

Ralph Eberhard Graichen, Singapore, SG;

Robert Zweigerdt, Singapore, SG;

Xiuqin Xu, Singapore, SG;

Christine Lindsay Mummery, DJ Bilthoven, NL;

William Sun, Singapore, SG;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 5/077 (2010.01); G01N 33/50 (2006.01); C12N 5/00 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); G01N 33/502 (2013.01); G01N 33/5061 (2013.01); A61K 35/12 (2013.01); C12N 5/0043 (2013.01); C12N 2500/25 (2013.01); C12N 2500/99 (2013.01); C12N 2501/02 (2013.01); C12N 2501/105 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/155 (2013.01); C12N 2501/33 (2013.01); C12N 2501/999 (2013.01); C12N 2502/13 (2013.01); C12N 2502/30 (2013.01); C12N 2506/02 (2013.01); C12N 2510/00 (2013.01);
Abstract

The present invention relates to the induction of differentiation in human embryonic stem (hES) cells to cardiomyocytes and factors such as prostaglandin I2 (PGI2), Fgf 9, Bmp6, Bmp4, Scf, Igf2, and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from hES cells is also provided wherein the media contains these factors. Genes that are upregulated in the process of cardiomyocyte differentiation are also provided.


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