The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 12, 2016

Filed:

May. 30, 2012
Applicants:

Fumiaki Watanabe, Kanagawa, JP;

Takanori Ambo, Aichi, JP;

Ai Hara, Kanagawa, JP;

Inventors:

Fumiaki Watanabe, Kanagawa, JP;

Takanori Ambo, Aichi, JP;

Ai Hara, Kanagawa, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/88 (2006.01); C12P 13/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/88 (2013.01); C12P 13/02 (2013.01); C12Y 402/01084 (2013.01); Y02P 20/52 (2015.11);
Abstract

Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.


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