The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2016

Filed:

Aug. 31, 2007
Applicants:

Ralf-holger Voss, Ingelheim, DE;

Matthias Theobald, Mainz-Kastel, DE;

Ratna Sari Intan Pöndl, Mainz, DE;

Renate Engel, Mainz, DE;

Simone Thomas, Mainz, DE;

Inventors:

Ralf-Holger Voss, Ingelheim, DE;

Matthias Theobald, Mainz-Kastel, DE;

Ratna Sari Intan Pöndl, Mainz, DE;

Renate Engel, Mainz, DE;

Simone Thomas, Mainz, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C12N 15/63 (2006.01); C12P 21/00 (2006.01); C07K 14/725 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/7051 (2013.01); C07K 16/00 (2013.01);
Abstract

The present invention relates to a method for producing a cell line expressing a stabilized functional single chain-antigen-recognizing genetic construct (scARC), comprising a genetic construct of the human scARC to be expressed, comprising the domains huV—Li-huV—Cand a genetic construct comprising the corresponding hetero-/(homo-)dimeric domain C, containing xenogenic, in its special case murine amino acid exchanges in the domains Cand C, wherein co-expression of the genetic constructs of the scARC-fragments occurs through the cell. Preferably, the scARCs are single chain-TCRs (scTCRs) or antibody-scFv-fragments, which further preferably recognize tumor associated peptide antigens (TAA). The present invention further relates to a gp-protein-specific T-cell response mediated α/β T-cell receptor rationally mutated by means of the method of the present invention and its uses.


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