The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 17, 2016

Filed:

May. 04, 2010
Applicants:

Lore Florin, Biberach an der Riss, DE;

Eric Becker, Hochdorf, DE;

Hitto Kaufmann, Ulm, DE;

Inventors:

Lore Florin, Biberach an der Riss, DE;

Eric Becker, Hochdorf, DE;

Hitto Kaufmann, Ulm, DE;

Assignee:

Boehringer Ingelheim International GmbH, Ingelheim am Rhein, DE;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 15/63 (2006.01); A61K 31/711 (2006.01); C12P 21/08 (2006.01); C07K 14/47 (2006.01);
U.S. Cl.
CPC ...
C07K 14/47 (2013.01);
Abstract

The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).


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