The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 29, 2016
Filed:
Jun. 08, 2011
Laura Sheard, Seattle, WA (US);
Ning Zheng, Shoreline, WA (US);
Ran Brosh, New York, NY (US);
Ihor R. Lemischka, Princeton, NJ (US);
Laura Sheard, Seattle, WA (US);
Ning Zheng, Shoreline, WA (US);
Ran Brosh, New York, NY (US);
Ihor R. Lemischka, Princeton, NJ (US);
University of Washington, Seattle, WA (US);
Abstract
Coronatine has been found to enhance binding of the JAZ1 degron to theF-box protein COI1, and analysis of the JAZ1 degron sequence has resulted in the identification of specific peptide sequences that bind COI1 with high affinity in the presence of coronatine. Crystal structure analysis has determined that coronatine and JA-Ile enhance the interaction between COI1 and JAZ1 via binding to a specific binding pocket on COI1. Attachment of one or more JAZ1 peptide tags as disclosed herein to a target protein in a non-plant cell expressingCOI1 or a homolog thereof results in degradation of the target protein following addition of a molecule that binds the coronatine/JA-Ile binding pocket on COI1. Therefore, provided herein are compositions, methods, and kits for targeted protein degradation.