The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2016
Filed:
Mar. 14, 2014
Applicant:
Wisconsin Alumni Research Foundation, Madison, WI (US);
Inventors:
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/069 (2013.01); C12N 2501/415 (2013.01); C12N 2506/02 (2013.01);
Abstract
Methods for generating human endothelial cells from human pluripotent stem cells under defined conditions in the absence of VEGF are described. Wnt/β-catenin signaling is activated in human pluripotent stem cells for a defined period, e.g., by inhibition of Gsk3, and then cultured without further exogenous activation of Wnt/β-catenin signaling to obtain a cell population containing human endothelial cells.