The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2016

Filed:

Apr. 07, 2013
Applicants:

Shandong Qihe Bio-technology Co., Ltd, Zibo, CN;

Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan, CN;

Inventors:

Qiang Yao, Jinan, CN;

Zhiyuan Gong, Jinan, CN;

Jianchang Su, Zibo, CN;

Zhaohui Liu, Jinan, CN;

Jilei Wang, Zibo, CN;

Xingcheng Du, Zibo, CN;

Xiao Liu, Jinan, CN;

Xiaoyan Zhu, Zibo, CN;

Qingxin Zhou, Jinan, CN;

Shan Zhu, Jinan, CN;

Yanwen Yang, Jinan, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 17/06 (2006.01); C12N 1/14 (2006.01); C12N 1/38 (2006.01);
U.S. Cl.
CPC ...
C12P 17/06 (2013.01); C12N 1/14 (2013.01); C12N 1/38 (2013.01);
Abstract

A method for increasing the yield of total flavonoids inmycelium by an expansin comprises: (1) inoculatinginto the PD liquid fermentation medium, activating the culturing of the strains and obtaining a seed solution; (2) inoculating the seed solution into a liquid fermentation medium, culturing and adding the expansin solution, then further culturing, isolating and obtainingmycelium; (3) extracting flavonoids from themycelium and obtaining total flavonoids. A plant expansin is used for the liquid fermentation production of flavonoids ingredients of, and the fermentation process and extraction method are optimized to increase greatly the yield of total flavonoids in


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