The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 15, 2016
Filed:
Jul. 29, 2011
Yukoh Hiei, Iwata, JP;
Yuji Ishida, Iwata, JP;
Yukoh Hiei, Iwata, JP;
Yuji Ishida, Iwata, JP;
JAPAN TOBACCO INC., Tokyo, JP;
Abstract
An object of the present invention is to provide a method of gene introduction, which can transform aplant at a higher efficiency compared to that in knownmethods, and a method of producing a transformedplant. The method of the invention includes a step of subjecting an immature embryo tissue of aplant to centrifugation treatment and/or pressurization treatment before the inoculation with, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 μM and/or a benzoic auxin in an amount of less than 5 μM, or not containing any phenoxy auxin and/or benzoic auxin.