The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2016

Filed:

Mar. 22, 2013
Applicant:

Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan, CN;

Inventors:

Qiang Yao, Jinan, CN;

Zhiyuan Gong, Jinan, CN;

Zhaohui Liu, Jinan, CN;

Jiandong Han, Jinan, CN;

Qi Wang, Jinan, CN;

Xiao Liu, Jinan, CN;

Luchang Wan, Jinan, CN;

Pengfei Ren, Jinan, CN;

Haixia Ren, Jinan, CN;

Jin Li, Jinan, CN;

Peng Yang, Jinan, CN;

Tao Sun, Jinan, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 1/14 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0061 (2013.01); C12N 1/14 (2013.01);
Abstract

A method for producing laccase by liquid fermentation ofcomprises the following steps of: (1) inoculating strains ofinto the PD liquid fermentation medium to perform activation culture and obtain a seed liquid; (2) inoculating the seed solution into a liquid fermentation medium to perform liquid fermentation culture, then adding an expansin solution to perform further culture, separating the supernatant to obtain a ferment solution; (3) extracting laccase from the ferment solution. A plant expansin and an optimized fermentation method are applied for producing laccase by liquid fermentation of, which increases the liquid fermentation output of laccase ofremarkably, by more than 3 times in comparing with traditional fermentation production methods.


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