The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 23, 2016
Filed:
Mar. 14, 2013
The Trustees of the Stevens Institute of Technology, Hoboken, NJ (US);
Hackensack University Medical Center, Hackensack, NJ (US);
Woo Lee, Ridgewood, NJ (US);
Jenny Zilberberg, Yardley, PA (US);
David Samuel Siegel, West Orange, NJ (US);
Peter Tolias, Westfield, NJ (US);
Hongjun Wang, Millburn, NJ (US);
Wenting Zhang, Jersey City, NJ (US);
The Trustees of the Stevens Institute of Technology, Hoboken, NJ (US);
Hackensack University Medical Center, Hackensack, NJ (US);
Abstract
The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.