The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 02, 2016
Filed:
Dec. 20, 2012
Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium
Jasmin Gielen, Bochum, DE;
Thomas Haas, Muenster, DE;
Hans-georg Hennemann, Marl, DE;
Steffen Schaffer, Herten, DE;
Mirja Wessel, Bochum, DE;
Katharina Berse, Waltrop, DE;
Marion Schiebelhut, Haltern, DE;
Sabine Hafkemeyer, Marl, DE;
Markus Poetter, Muenster, DE;
Frank Erhardt, Bielefeld, DE;
Jasmin Gielen, Bochum, DE;
Thomas Haas, Muenster, DE;
Hans-Georg Hennemann, Marl, DE;
Steffen Schaffer, Herten, DE;
Mirja Wessel, Bochum, DE;
Katharina Berse, Waltrop, DE;
Marion Schiebelhut, Haltern, DE;
Sabine Hafkemeyer, Marl, DE;
Markus Poetter, Muenster, DE;
Frank Erhardt, Bielefeld, DE;
EVONIK DEGUSSA GmbH, Essen, DE;
Abstract
A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.