The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 02, 2016
Filed:
Sep. 08, 2011
Chengzao Sun, San Diego, CA (US);
Behrouz Bruce Forood, San Diego, CA (US);
Gary Luo, San Diego, CA (US);
Soumitra S. Ghosh, San Diego, CA (US);
Chengzao Sun, San Diego, CA (US);
Behrouz Bruce Forood, San Diego, CA (US);
Gary Luo, San Diego, CA (US);
Soumitra S. Ghosh, San Diego, CA (US);
Amylin Pharmaceuticals, LLC, Wilmington, DE (US);
AstraZeneca Pharmaceuticals LP, Wilmington, DE (US);
Abstract
There are provided compounds and methods for amidating the C-terminus of a polypeptide. The methods include reacting a polypeptide which includes a C-terminal thioester or C-terminal selenoester with any one of a defined set of auxiliary molecules under conditions suitable to produce a polypeptide adduct which includes the auxiliary molecule chemically bound at the C-terminal of the polypeptide. The auxiliary molecule can be a substituted or unsubstituted 2-phenyl-2-amino ethanethiol, a substituted or unsubstituted 2-phenyl-2-amino ethaneselenol, or a substituted xanthene. In the subsequent step, a portion of the auxiliary molecule is removed from the C-terminal of the polypeptide adduct and leaving the amide nitrogen under conditions suitable to form a C-terminal free amide polypeptide.