The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 15, 2015

Filed:

Jun. 15, 2012
Applicants:

Sanford Jay Silverman, Roosevelt, NJ (US);

Robert Scott Mcisaac, Princeton, NJ (US);

Marcus Noyes, Monmouth Junction, NJ (US);

David Botstein, Princeton, NJ (US);

Inventors:

Sanford Jay Silverman, Roosevelt, NJ (US);

Robert Scott McIsaac, Princeton, NJ (US);

Marcus Noyes, Monmouth Junction, NJ (US);

David Botstein, Princeton, NJ (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/79 (2006.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1079 (2013.01); C12N 15/63 (2013.01); C12N 15/81 (2013.01);
Abstract

A system allows for rapid and specific induction of individual genes in eukaryotic cells using a chimeric transcriptional activator that is responsive to hormone inducer. Upon addition of the hormone, cytoplasmic transcriptional activator localizes to the nucleus and subsequently binds to promoters containing sequences that bind to its DNA-binding domain. Genetic modifications allow for rapid and specific degradation of a targeted protein upon addition of hormone by means of a regulated degron method that utilizes a protease variant. This system is useful for discovering new compounds by high throughput screening when introducing compound libraries to these protein-depleted cells.


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