The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 29, 2015
Filed:
Mar. 15, 2013
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
George R. Feehery, West Newbury, MA (US);
Fiona Stewart, Ipswich, MA (US);
James McFarland, Beverly, MA (US);
Sriharsa Pradhan, Wenham, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12Q 1/6806 (2013.01); C12Q 2522/101 (2013.01); C12Q 2525/117 (2013.01); C12Q 2527/125 (2013.01); C12Q 2527/137 (2013.01); C12Q 2537/164 (2013.01);
Abstract
Compositions and methods are provided for enriching mitochondrial DNA and optionally chloroplast DNA from eukaryotic cells in a simple rapid method that provides greater than 100 fold enrichment. Affinity protein-coated substrate in a buffer is used to efficiently bind chromosomal DNA and thereby remove it from the buffer. Mitochondrial sequencing reads reveal that non-biased sequence selection providing representation of a substantial proportion of mitochondrial DNA in the eukaryotic cells analyzed.